Journal: BMC Pulmonary Medicine

Article Title: β-elemene inhibits tumor-promoting in small cell lung cancer by affecting M2 macrophages and TGF-β

doi: 10.1186/s12890-025-03533-z

Figure Lengend Snippet: The Effect of M2 Macrophages on NCI-H209 Cells. ( A ) PMA-induced differentiation of THP-1 into M0 macrophages. ( B and C ) IL-4 and IL-13 induce the differentiation of M0 macrophages into M2 macrophages. ( D ) CCK8 assay to detect the effect of M2 macrophages on the viability of NCI-H209 cells. ( E ) Flow cytometry to analyze the cell cycle distribution of NCI-H209 cells treated with M2 macrophages. ( F ) Flow cytometry to analyze the cell apoptosis of NCI-H209 cells treated with M2 macrophages. ( G ) Transwell assays to examine the effect of M2 macrophages on the migration of NCI-H209 cells. Scale bar = 100 μm. ( H ) ELISA assay to detect the effect of M2 macrophages on TGF-β expression. ** p < 0.01, *** p < 0.001, ns” stands for “no significant difference

Article Snippet: Subsequently, the cells were incubated with THP-1 cell-specific culture medium (CM-0233, Procell, Wuhan, China) containing IL-4 (20 ng/mL) (HY-P700130AF, MCE, Shanghai, China) and IL-13 (5 ng/mL) (HY-P70568, MCE, Shanghai, China) for 48 h to polarize them into M2 macrophages.

Techniques: CCK-8 Assay, Flow Cytometry, Migration, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Current Issues in Molecular Biology

Article Title: Transcriptomic Establishment of Pig Macrophage Polarization Signatures

doi: 10.3390/cimb45030151

Figure Lengend Snippet: Brief methods of bone marrow-derived macrophage induction.

Article Snippet: M2_IL4 + IL10 , M2 , 10 ng/mL IL10(Proteintech, USA, HZ-1145), and 10 ng/mL IL4 (Proteintech, Rosemount, IL, USA, HZ-1004).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Transcriptomic Establishment of Pig Macrophage Polarization Signatures

doi: 10.3390/cimb45030151

Figure Lengend Snippet: Transcriptomic comparison of porcine macrophage phenotypes, M1 (M1_IFNγ + LPS, and M1_GM-CSF) versus M2 (M1_IFNγ + LPS, and M1_GM-CSF). ( A ) PCA clustering M1_IFNγ + LPS ( n = 4), M1_GM-CSF ( n = 4), M2_IL4 + IL10 ( n = 3), M2_M-CSF ( n = 3); ( B ) Top enriched GO biological processes of 730 DEG genes, M1 (M1_IFNγ + LPS, and M1_GM-CSF) and M2 (M2_IL4 + IL10, and M2_M-CSF); ( C ) Heatmap exhibiting 49 classical macrophage marker genes expression profile among M1_IFNγ + LPS, M1_GM-CSF, M2_IL4 + IL10, and M2_M-CSF; ( D ) The DEGs between M1 and M2 were studied by using the String online tool. The interaction between each protein pair is represented by lines, and the size of the circle is directly proportional to the degree.

Article Snippet: M2_IL4 + IL10 , M2 , 10 ng/mL IL10(Proteintech, USA, HZ-1145), and 10 ng/mL IL4 (Proteintech, Rosemount, IL, USA, HZ-1004).

Techniques: Comparison, Marker, Expressing

Journal: Current Issues in Molecular Biology

Article Title: Transcriptomic Establishment of Pig Macrophage Polarization Signatures

doi: 10.3390/cimb45030151

Figure Lengend Snippet: Comparison of transcriptomics in different phenotypes of porcine macrophages. ( A ) Heatmap showing that specific macrophage phenotypes were aggregated by the induction method, M1_IFNγ + LPS, and M1_GM-CSF; ( B ) Heatmap showing that specific macrophage phenotypes were aggregated by the induction method M2_IL4 + IL10 and M2_M-CSF; ( C ) GO pathway enrichment analysis of two polarized macrophages, M1_IFNγ + LPS and M1_GM-CSF; ( D ) GO pathway enrichment analysis of two polarized macrophages, M2_IL4 + IL10 and M2_M-CSF; ( E ) The DEGs were studied by using the String online tool, M1_IFNγ + LPS, and M1_GM-CSF. The interaction between each protein pair is represented by lines, and the size of the circle is directly proportional to the degree of interaction. We adopt a node number greater than 1; ( F ) The DEGs are studied by using the String online tool, M2_IL4 + IL10, and M2_M-CSF. The interaction between each protein pair is represented by lines, and the size of the circle is directly proportional to the degree of interaction. We adopt a node number greater than 2.

Article Snippet: M2_IL4 + IL10 , M2 , 10 ng/mL IL10(Proteintech, USA, HZ-1145), and 10 ng/mL IL4 (Proteintech, Rosemount, IL, USA, HZ-1004).

Techniques: Comparison

Journal: Current Issues in Molecular Biology

Article Title: Transcriptomic Establishment of Pig Macrophage Polarization Signatures

doi: 10.3390/cimb45030151

Figure Lengend Snippet: Validation of M1_IFNγ + LPS, M1_GMCSF, M2_M-CSF, and M2_IL4 + IL10 signatures. ( A ) SS2-infected macrophages were enriched in M1_IFNγ + LPS based on GSEA (Data source: Microarray analysis); ( B ) PRRSV-infected macrophages were enriched in M1_GM-CSF based on GSEA (Data source: RNA-seq); ( C ). T. gondii -infected macrophages were enriched in M2_IL4 + IL10 based on GSEA (Data source: RNA-seq); ( D ) T. gondii Me49 -infected macrophages were enriched in M2_M-CSF based on GSEA (Data source: RNA-seq).

Article Snippet: M2_IL4 + IL10 , M2 , 10 ng/mL IL10(Proteintech, USA, HZ-1145), and 10 ng/mL IL4 (Proteintech, Rosemount, IL, USA, HZ-1004).

Techniques: Biomarker Discovery, Infection, Microarray, RNA Sequencing

Journal: Veterinary immunology and immunopathology

Article Title: Interleukin-4 (IL-4) may regulate alternative activation of macrophage-like cells in chickens: A sequential study using novel and specific neutralizing monoclonal antibodies against chicken IL-4.

doi: 10.1016/j.vetimm.2018.10.011

Figure Lengend Snippet: Fig. 1. Detection of recombinant yeast chIL-4 by western blot analysis. (A) Western blot detection of yeast chIL-4 using mouse anti-chIL-4 monoclonal antibody #6D9G and cross reactivity of #6D9G with mouse and human IL-4 as well as chicken IL-13, IL-10 and IFN-γ, (B) Blot showing detection of chIL-4 using mouse anti- chIL-4 monoclonal antibody #8GE10 and cross reactivity of ##8GE10 with mouse and human IL-4 as well as chicken IL-13, IL-10 and IFN-γ, (C) chIL-4 detection using rabbit polyclonal antibody #53 against chIL-4. Molecular weight (kDa) marker is presented on the left. Data represent three independent experiments.

Article Snippet: Similarly, the cross reactivity of these mAbs was determined using mouse and human IL-4 as well as chicken chIL-13, chIL-10 and chIFN-γ at a concentration of 1 μg/lane (all from Kingfisher biotech, St. Paul, MN, USA).

Techniques: Recombinant, Western Blot, Molecular Weight, Marker